chicken ova 323–339 peptide (h-2 d restricted; amino acid sequence isqavhaahaeineagr)  (GenScript corporation)

Journal: Cells

Article Title: Dendritic Cell-Based Vaccines Recruit Neutrophils to the Local Draining Lymph Nodes to Prime Natural Killer Cell Responses

doi: 10.3390/cells12010121

Figure Lengend Snippet: Neutrophils did not influence dendritic (DC) vaccine-mediated education of antigen-specific CD8+ T cell degranulation or production of interferon (IFN γ ) and tumor necrosis factor (TNF) α . Female C57BL/6 mice (n = 12–16 per treatment) were given anti-Ly6G followed by DC vaccines. One week later, the spleens were examined for CD8+ T cell responses to stimulation with the vaccine-loaded epitopes, ovalbumin (OVA) 257-264 (SIIN), which is the immunodominant CD8+ T cell epitope, and OVA 323-339 (ISQ), which is the dominant CD4+ helper T cell epitope. The CD8+ T cell responses were measured by expression of CD107a as an indicator of degranulation, and production of IFN γ and TNF α using flow cytometry. The CD8+ T cell responses in the spleens of mice that received anti-Ly6G and the DC vaccine, the DC vaccine only, PBS only, and anti-Ly6G and PBS were compared using a two-way analysis of variance; p -value < 0.05 (*), p -value < 0.005 (**), p -value < 0.001 (***), p -value < 0.0001 (****). Graphs show the means and the standard deviation with each dot representing an individual mouse.

Article Snippet: The cells were stimulated for one hour with lipopolysaccharide (LPS) from Escherichia coli O55:B5 (Sigma, Oakville, Canada, Cat#L2880) at a concentration of 100 ng/mL and 1 µg/mL of both chicken ovalbumin (OVA) 257-264 (SIINFEKL or SIIN) and chicken and OVA 323-339 (ISQAVHAAHAEINEA or ISQ) peptides (both from PepScan Systems, Lelystad, The Netherlands), which are immunodominant epitopes for CD8 + and CD4 + T cells, respectively, in mice with major histocompatibility complex haplotype ‘b’.

Techniques: Vaccines, Expressing, Flow Cytometry, Standard Deviation